念珠菌体外药敏试验方法的比较

目的探讨酵母菌纸片扩散法的临床适用性.方法分别采用E-test法、法国梅里埃ATB Fungs酵母菌药敏试验与K-B纸片法,检测念珠菌的体外药物敏感率,比较其差异显著性.结果 K-B纸片法和E-test法同时监测伊曲康唑(IT)、氟康唑(FL).2种药物的符合率为96.8%、100%,其敏感率比较88%/85%、98.4%/98.4%,差异无显著性(P＞0.05);K-B纸片法和法国梅里埃ATB Fungs酵母菌药敏试验法对制霉菌素(NYS)、酮康唑(KET)、益康唑(ECO)和咪康唑(MIC)这4种药物药敏结果的符合率分别为100%、100%、91.2%和93.6%,敏感率比较100%/100%、83.2%/83.2%、70%/62%和68%/71%,差异也无显著性(P＞0.05).结论 K-B纸片扩散法可用于临床酵母菌的药敏试验.

The comparison of the methods for drug sensitivity test of Candida

Objective To surrey the clinical value of drug sensitivity test of Canchda by Kirby-Bauer paper flakes diffusiion method.Methods E-test assay,ATB Fungus bioMeieux France and kirby-Bauer paper flakes diffusion difference of these three methods.Results To compare the suseeptivity rate of K-B paper and E-test in two drugs itraconazole(IT) and fluconazole(FL),the result showed that there was no significance difference (P>0.05,88%/85%,98.4%/98.4%) between two methods.Comparing the susceptivity rate of K-B paperand ATB Fungus in four drugs nystatin(NYS),ketoconazole(KET),enconazole(ECO)and miconazole(MIC),there was no significance difference also(P>0.05,100%/100%,83.2%/83.2%,70%/62%,68%/71%).Conclusion Kirby-Bauer paper flakes diffusion method could be used in the drug sensitivity test of Candida.