Characterization of a CENP-B homolog in the holocentric Lepidoptera Spodoptera frugiperda |
| |
| Authors: | d'Alençon Emmanuelle Nègre Nicolas Stanojcic Slavica Alassoeur Benjamin Gimenez Sylvie Léger Alexandre Abd-Alla Adly Juliant Sylvie Fournier Philippe |
| |
| Institution: | a UMR1333 INRA, Université Montpellier II, Place Eugène Bataillon, 34095 Montpellier Cedex 5, Franceb UPR1142 CNRS, Institute of Human Genetics, Montpellier Cedex, Francec Department of Pests and Plant Protection, National Research Center, Dokki, Giza, Egyptd UPS 3044, CNRS, Avenue du Général de Gaulle 30380 Saint Christol les Alès, France |
| |
| Abstract: | The discovery of an homolog of the human centromeric protein B, CENP-B, in an EST database of the holocentric insect species Spodoptera frugiperda prompted us to further characterize that gene because i) CENP-B has not been described in invertebrates yet ii) it should be a milestone in the molecular characterization of the holocentric centromere of Lepidoptera.Like its human counterpart, the Sf CENP-B protein is related to the transposase of the pogo transposable element (TE) of D. melanogaster. In this paper, we show evidences that the lepidopteran cenpB gene has evolved from domestication of a transposase. Furthermore, the Sf CENP-B nuclear location and its ability to bind to a retrotransposon derived sequence in vivo argue in favor of a functional homology to CENP-B proteins. |
| |
| Keywords: | LTR Long Terminal Repeat ACA anti-centromere antibodies TBP TATA box Binding Protein ChIP Chromatin Immunoprecipitation TE transposable element |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
