Molecular cloning and polymorphism analysis of the prion protein gene in Tan sheep of Ningxia, China |
| |
Authors: | Xu Lihua Zhang Zhuming Zhou Xiangmei Yin Xiaomin Yang Lifeng Zhao Deming |
| |
Institution: | a State Key Laboratories for Agrobiotechnology, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, Chinab College of Agriculture, Ningxia University, Yinchuan 750021, China |
| |
Abstract: | The resistance or susceptibility of sheep to scrapie is associated with polymorphisms of the prion protein gene (PRNP), particularly, single nucleotide polymorphisms (SNPs) in amino acid positions 136, 154 and 171. The prion protein (PrP) gene sequence and the deduced amino acid alignment of prion protein in Tan sheep, a local Chinese sheep breed traditionally raised in Ningxia, northwestern China, were determined and variability of the PrP amino acids sequence was analyzed in this study. The PrP nucleic acids and amino acids sequences of 112 Tan sheep were highly homogenous, although polymorphism of the PrP gene was detected at several sites, particularly codons 106, 154, and 171. The analysis of both sequences revealed that the most predominant allele at codons 136, 154 and 171 in Tan sheep was ARQ, which was known to be associated with high susceptibility to scrapie in sheep. The result suggests that Tan sheep is potentially susceptible to scrapie. Our findings provide valuable information for future breeding projects to scrapie resistance in Tan sheep. |
| |
Keywords: | TSE transmissible spongiform encephalopathies CJD Creutzfeldt-Jakob disease BSE bovine spongiform encephalopathy PrP prion protein PrPc cellular prion protein PRNP prion protein gene SNPs single nucleotide polymorphisms ARQ A136R154Q171 ARR A136R154R171 AHQ A136H154Q171 ARH A136R154H171 VRQ V136R154Q171 PCR polymerase chain reaction |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|