Fingerprint of Biomphalaria arabica, the intermediate host of Schistosoma mansoni in Saudi Arabia, using RAPD-PCR |
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Authors: | Bin Dajem Saad M Ibrahim Essam H Al-Quraishy Saleh A S Mostafa Osama M S |
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Institution: | a Biology Department, Faculty of Science, King Khaled University, Abha, P.O Box 9004, Saudi Arabiab Blood Products Quality Control and Research Department, National Organization for Research and Control of Biologicals, Cairo, Egyptc Department of Zoology, Faculty of Science, King Saud University, Saudi Arabiad Zoology Department, Faculty of Science, Ain Shams University, Abbassia 11566, Cairo, Egypt |
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Abstract: | In the time schistosomisis control programs are implemented in many countries, schistosomiasis continues to spread throughout the world. Among these control strategies is the vector control. Within this context, analysis of the genetic variability of the intermediate host snails is important because it allows identification of specific sequences of the genome of this mollusk related to determine their fingerprint. We investigated Biomphalaria arabica, which is found in Saudi Arabia, the intermediate host of Schistosoma mansoni infection. Genetic fingerprint was studied by RAPD-PCR using our own different random primers as well as published primers. The electrophoretic patterns resulting from amplification showed specific polymorphic markers of B. arabica. This information will be helpful in the identification of the snails and demonstrating that RAPD-PCR is an appropriate and efficient methodological approach for establishment of genetic barcode development. |
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Keywords: | B Arabica Biomphalaria arabica ITS internal transcribed spacer KSA Kingdom of Saudi Arabia rDNA nuclear ribosomal DNA RAPD randomly amplified polymorphic RFLP restriction fragment length polymorphism Ribonuclease A RNase A rRNA ribosomal RNA S haematobium Schistosoma haematobium S japonicum Schistosom japonicum S mansoni Schistosom mansoni |
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