The multi-PDZ domain protein-1 (MUPP-1) expression regulates cellular levels of the PALS-1/PATJ polarity complex |
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Authors: | Emeline Assémat Emmanuelle Crost Marion Ponserre Jan Wijnholds Andre Le Bivic Dominique Massey-Harroche |
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Institution: | 1. Aix-Marseille Université, CNRS, IBDM UMR7288, 13288 Marseille, France;2. Department of Neuromedical Genetics, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences, 1105 BA Amsterdam, The Netherlands |
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Abstract: | MUPP-1 (multi-PDZ domain protein-1) and PATJ (PALS-1-associated tight junction protein) proteins are closely related scaffold proteins and bind to many common interactors including PALS-1 (protein associated with Lin seven) a member of the Crumbs complex. Our goal is to understand how MUPP-1 and PATJ and their interaction with PALS-1 are regulated in the same cells. We have shown that in MCF10A cells there are at least two different and co-existing complexes, PALS-1/MUPP-1 and PALS-1/PATJ. Surprisingly, MUPP-1 levels inversely correlated with PATJ protein levels by acting on the stabilization of the PATJ/PALS-1 complex. Upon MUPP-1 depletion, the increased amounts of PATJ are in part localized at the migrating front of MCF10A cells and are able to recruit more PAR3 (partition defective 3). All together these data indicate that a precise balance between MUPP-1 and PATJ is achieved in epithelial cells by regulating their association with PALS-1. |
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Keywords: | MUPP-1 multi-PDZ domain protein-1 PATJ PALS-1-associated tight junction protein PALS protein associated with Lin seven PAR partition defective PDZ acronym combining the first letters of three proteins: post synaptic density protein (PSD95) Dlg1 Drosophila disc large tumor suppressor ZO 1 zonula occludens-1 protein CRB crumbs L27 Lin2/lin7 domain TJ tight junction aPKC atypical protein kinase C |
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