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Determination of topological structure of ARL6ip1 in cells: Identification of the essential binding region of ARL6ip1 for conophylline |
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| Authors: | Masahiro Kuroda Shintaro Funasaki Tsuyoshi Saitoh Yukiko Sasazawa Shigeru Nishiyama Kazuo Umezawa Siro Simizu |
| Institution: | 1. Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan;2. Department of Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan;3. Department of Molecular Target Medicine Screening, Aichi Medical University School of Medicine, Nagakute, Japan |
| Abstract: | Conophylline (CNP) has various biological activities, such as insulin production. A recent study identified ADP-ribosylation factor-like 6-interacting protein 1 (ARL6ip1) as a direct target protein of CNP. In this study, we revealed that ARL6ip1 is a three-spanning transmembrane protein and determined the CNP-binding domain of ARL6ip1 by deletion mutation analysis of ARL6ip1 with biotinyl-amino-CNP. These results suggest that CNP is expected to be useful for future investigation of ARL6ip1 function in cells. Because of the anti-apoptotic function of ARL6ip1, CNP may be an effective therapeutic drug and/or a novel chemosensitizer for human cancers and other diseases. |
| Keywords: | ARL6ip1 ADP-ribosylation factor-like 6-interacting protein 1 CNP conophylline BCNP biotinyl-aminoconophylline ER endoplasmic reticulum Gluc Gaussia luciferase TM transmembrane |
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