LMO4 modulates proliferation and differentiation of 3T3-L1 preadipocytes |
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Authors: | Ning Wang Xichen WangMingxin Shi Hongyan ShiXiaohong Yan Hui LiShouzhi Wang Yuxiang Wang |
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Affiliation: | College of Animal Science and Technology, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, Heilongjiang 150030, China |
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Abstract: | Previous microarray analyses revealed that LMO4 is expressed in 3T3-L1 preadipocytes, however, its roles in adipogenesis are unknown. In the present study, using RT-PCR sequencing and quantitative real-time RT-PCR, we confirmed that LMO4 gene is expressed in 3T3-L1 preadipocytes and its expression peaks at the early stage of 3T3-L1 preadipocyte differentiation. Further analyses showed that LMO4 knockdown decreased the proliferation of 3T3-L1 preadipocytes, and attenuated the differentiation of 3T3-L1 preadipocytes, as evidenced by reduced lipid accumulation and down-regulation of PPARγ gene expression. Collectively, our findings indicate that LMO4 is a novel modulator of adipogenesis. |
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Keywords: | LMO4 3T3-L1 preadipocyte Cell proliferation Preadipocyte differentiation |
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