MicroRNAs with analogous target complementarities perform with highly variable efficacies in Arabidopsis |
| |
Authors: | Ira Deveson Junyan LiAnthony A Millar |
| |
Institution: | Research School of Biology, Australian National University, 0200 ACT, Australia |
| |
Abstract: | In plants, the silencing efficacy of microRNAs (miRNAs) is thought to be predominantly determined by the degree of complementarity to their target genes. Here, silencing efficacy was determined for Arabidopsis miR159 and four artificial miRNAs (amiRNAs) that all target MYB33/MYB65 with analogous complementarities. As determined through complementation of a loss-of-function mir159 mutant, the amiRNAs displayed highly variable efficacies, none of which was as strong as endogenous miR159. This was despite amiRNA expression levels being many fold-higher than miR159 in wild-type. The results highlight the variable nature of miRNA silencing efficacy in plants, where it appears that factors additional to complementarity strongly impact silencing. |
| |
Keywords: | miR159 MYB33/65 amiRNAs Efficacy Gene silencing miRNA abundance Arabidopsis |
本文献已被 ScienceDirect 等数据库收录! |