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Cyanide-induced death of cells in plant leaves
Authors:L. A. Vasil’ev  A. A. Vorobyov  E. V. Dzyubinskaya  A. V. Nesov  A. A. Shestak  V. D. Samuilov
Affiliation:(1) Department of Physiology of Microorganisms, Faculty of Biology, Lomonosov Moscow State University, 119992 Moscow, Russia
Abstract:Destruction of guard cell nuclei in epidermis isolated from leaves of pea, maize, sunflower, and haricot bean, as well as destruction of cell nuclei in leaves of the aquatic plants waterweed and eelgrass were induced by cyanide. Destruction of nuclei was strengthened by illumination, prevented by the antioxidant alpha-tocopherol and an electron acceptor N,N,N ,N -tetramethyl-p-phenylenediamine, and removed by quinacrine. Photosynthetic O2 evolution by the leaf slices of a C3 plant (pea), or a C4 plant (maize) was inhibited by CN- inactivating ribulose-1,5-bisphosphate carboxylase, and was renewed by subsequent addition of the electron acceptor p-benzoquinone.
Keywords:programmed cell death  cyanide  quinacrine  leaf cells  light microscopy   Pisum sativum L.   Helianthus annuus L.   Phaseolus vulgaris L.   Zea mays L.   Elodea canadensis Michx.   Vallisneria spiralis L.
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