Use of (1-14C) aectic anhydride to quantitate diglycerides: a new analytical procedure |
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| Authors: | M W Banschbach R L Geison J F O'Brien |
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| Affiliation: | Neurometabolism Section, Waisman Center on Mental Retardation, University of Wisconsin, Madison, Wisconsin 53706 USA |
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| Abstract: | A microanalytical procedure is described for perchloric acid-catalyzed acetylation of diglycerides in pyridine using [1-14C]acetic anhydride of known specific activity. Incorporated [1-14C]acetate permits the rapid, accurate, and nondestructive quantitation of diglycerides in the concentration range of 0.005 μmoles to 1 μmole. Diglyceride is completely acetylated within 20–25 min at room temperature. No intermolecular rearrangements are observed; some intramolecular rearrangement does occur. |
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