Phloem loading in Vicia faba leaves: Effect of N-ethylmaleimide and parachloromercuribenzenesulfonic acid on H+ extrusion,K+ and sucrose uptake |
| |
Authors: | Serge Delrot Jean-Pierre Despeghel Jean-Louis Bonnemain |
| |
Institution: | (1) Laboratoire de Biologie et Physiologie Végétales, Université de Poitiers, 25 Faubourg Saint Cyprien, F-86000 Poitiers, France |
| |
Abstract: | The effects of a penetrating (NEM) and a non-penetrating (PCMBS) sulfhydryl-specific reagent on proton extrusion, 86Rb and U-14C]sucrose uptake by Vicia faba leaves have been studied. Proton extrusion was strongly or completely inhibited by 0.1 mM NEM. 86Rb and U-14C]sucrose uptake were markedly reduced by NEM concentrations equal to or higher than 0.5 mM. Under our experimental conditions, PCMBS (1 mM) exerted a strong inhibition on 14C]sucrose uptake but did not inhibit proton extrusion and 86Rb uptake. The sensitivity of phloem loading to PCMBS is thought to be a consequence of sugar-carrier blockage and not of inhibition of the proton pump.Abbreviations CCCP
carbonylcyanide-m-chlorophenylhydrazone
- DES
diethylstilbestrol
- DCCD
dicyclohexylcarbodiimide
- FC
Fusicoccin
- NEM
N-ethylmaleimide
- PCMBS
p-chloromercuribenzenesulfonic acid |
| |
Keywords: | Phloem loading Potassium uptake Proton extrusion Sucrose uptake Sulfhydryl-specific reagents Uptake (H+ K+ sucrose) Vicia |
本文献已被 SpringerLink 等数据库收录! |