Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain |
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Authors: | Makoto Morita Hidemitsu Sugihara Kazuhiro Tokunaka Arihiro Tomura Kan Saiga Takamichi Sato Yasutada Imamura Toshihiko Hayashi |
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Affiliation: | 1. Pharmaceutical Research Laboratories, Nippon Kayaku Co., Ltd., Tokyo 115-8588, Japan;2. Department of Chemistry and Life Science, School of Advanced Engineering, Kogakuin University, Hachioji, Tokyo 192-0015, Japan;3. China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China |
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Abstract: | This report describes the preparation and partial characterization of monoclonal antibodies that are reactive specifically with the nascently produced non-triple helical form of the type IV collagen α1 chain, designated as NTH α1(IV). These antibodies were nonreactive with the α1 chain of the type IV collagen in the triple-helical conformation. Three antibodies, #141, #179 and #370, with different epitopes in NTH α1(IV) were found to be reactive with the nascent polypeptide secreted from human normal cells and a human carcinoma cell line. The antibodies with different epitopes may provide a key method for elucidating the physiological function and tissue distribution of NTH α1(IV), which is distinct from the chain derived from triple-helical type IV collagen. |
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Keywords: | Type IV collagen Triple-helical conformation Antibody Basement membrane Hydroxyproline |
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