Proteomic analysis of bone proteins adsorbed onto the surface of titanium dioxide |
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| Authors: | Keisuke Sugimoto Shuhei Tsuchiya Masahiro Omori Ryo Matsuda Masahito Fujio Kensuke Kuroda Masazumi Okido Hideharu Hibi |
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| Affiliation: | 1. Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan;2. Nagoya University Hospital Oral and Maxillofacial Surgery, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan;3. EcoTopia Science Institute, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8603, Japan |
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| Abstract: | Osseointegration is the structural and functional connection between bone tissues and implants such as titanium dioxide (TiO2). The bone-TiO2 interface is thought to contain proteoglycans. However, exhaustive analysis of the proteins in this layer has not been performed. In this study, we evaluated the bone protein adhered on the surface of TiO2 comprehensively. Pig bone protein was extracted by sequential elutions with guanidine, 0.1 M EDTA, and again with guanidine. The proteins obtained from these extractions were allowed to adhere to an HPLC column packed with TiO2 and were eluted with 0.2 M NaOH. The eluted proteins were identified by LC/MS/MS and included not only proteoglycans but also other proteins such as extracellular matrix proteins, enzymes, and growth factors. Calcium depositions were observed on TiO2 particles incubated with bone proteins, guanidine-extracted proteins adhered to TiO2 displayed significantly high amounts of calcium depositions. |
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| Keywords: | Titanium dioxide Proteomics Osseointegration Bone protein Protein adsorption |
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