Genome-Scale Validation of Deep-Sequencing Libraries |
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Authors: | Dominic Schmidt Rory Stark Michael D Wilson Gordon D Brown Duncan T Odom |
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Institution: | 1. Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Cambridge, United Kingdom.; 2. Department of Oncology, Hutchison/MRC Research Centre, Cambridge, United Kingdom.;Wellcome Trust Sanger Institute, United Kingdom |
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Abstract: | Chromatin immunoprecipitation followed by high-throughput (HTP) sequencing (ChIP-seq) is a powerful tool to establish protein-DNA interactions genome-wide. The primary limitation of its broad application at present is the often-limited access to sequencers. Here we report a protocol, Mab-seq, that generates genome-scale quality evaluations for nucleic acid libraries intended for deep-sequencing. We show how commercially available genomic microarrays can be used to maximize the efficiency of library creation and quickly generate reliable preliminary data on a chromosomal scale in advance of deep sequencing. We also exploit this technique to compare enriched regions identified using microarrays with those identified by sequencing, demonstrating that they agree on a core set of clearly identified enriched regions, while characterizing the additional enriched regions identifiable using HTP sequencing. |
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