Plasma membrane temperature gradients and multiple cell permeabilization induced by low peak power density femtosecond lasers |
| |
Authors: | Allen L. Garner V. Bogdan Neculaes Maxim Deminsky Dmitry V. Dylov Chulmin Joo Evelina R. Loghin Siavash Yazdanfar Kenneth R. Conway |
| |
Affiliation: | 1. School of Nuclear Engineering, Purdue University, West Lafayette, IN 47907, USA;2. GE Global Research Center, 1 Research Circle, Niskayuna, NY 12309, USA;3. Kintech LTD, Kurchatov sq. 1, 123182 Moscow, Russia;4. NRC “Kurchatov Institute”, Kurchatov sq. 1, 123182 Moscow, Russia;5. School of Mechanical Engineering, Yonsei University, Seoul 120-749, South Korea |
| |
Abstract: | Calculations indicate that selectively heating the extracellular media induces membrane temperature gradients that combine with electric fields and a temperature-induced reduction in the electropermeabilization threshold to potentially facilitate exogenous molecular delivery. Experiments by a wide-field, pulsed femtosecond laser with peak power density far below typical single cell optical delivery systems confirmed this hypothesis. Operating this laser in continuous wave mode at the same average power permeabilized many fewer cells, suggesting that bulk heating alone is insufficient and temperature gradients are crucial for permeabilization. This work suggests promising opportunities for a high throughput, low cost, contactless method for laser mediated exogenous molecule delivery without the complex optics of typical single cell optoinjection, for potential integration into microscope imaging and microfluidic systems. |
| |
Keywords: | Laser Optoporation Thermal gradients Electropermeabilization Transfection |
本文献已被 ScienceDirect 等数据库收录! |
|