Identification of the agg1 mutation responsible for negative phototaxis in a “wild-type” strain of Chlamydomonas reinhardtii |
| |
| Authors: | Takahiro Ide Shota Mochiji Noriko Ueki Katsushi Yamaguchi Shuji Shigenobu Masafumi Hirono Ken-ichi Wakabayashi |
| |
| Institution: | 1. Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, Japan;2. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan;3. Department of Biological Sciences, Graduate School of Science and Engineering, Chuo University, Tokyo 112-8551, Japan;4. Functional Genomics Facility, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan;5. Department of Basic Biology, Faculty of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Aichi 444-8585, Japan;6. Department of Frontier Bioscience, Hosei University, Tokyo 184-8584, Japan |
| |
| Abstract: | The unicellular green alga Chlamydomonas reinhardtii is a model organism for various studies in biology. CC-124 is a laboratory strain widely used as a wild type. However, this strain is known to carry agg1 mutation, which causes cells to swim away from the light source (negative phototaxis), in contrast to the cells of other wild-type strains, which swim toward the light source (positive phototaxis). Here we identified the causative gene of agg1 (AGG1) using AFLP-based gene mapping and whole genome next-generation sequencing. This gene encodes a 36-kDa protein containing a Fibronectin type III domain and a CHORD-Sgt1 (CS) domain. The gene product is localized to the cell body and not to flagella or basal body. |
| |
| Keywords: | Phototaxis Next-generation sequence |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
