Evaluation of different culture conditions ofClostridium bifermentans DPH-1 for cost effective PCE degradation |
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Authors: | Afroze Syeda Humayra Yuki Hasegawa Izumi Nomura Young C Chang Takeshi Sato Kazuhiro Takamizawa |
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Institution: | (1) United Graduate School of Agricultural Science, Gifu University, 501-1193 Gifu, Japan;(2) Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University, 501-1193 Gifu, Japan;(3) Department of Civil Engineering, Gifu University, 501-1193 Gifu, Japan |
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Abstract: | Clostridium bifermentans strain DPH-1 has already been found to dechlorinate perchloroethylene (PCE) tocis-dichloroethylene (cis-DCE)via trichloroethylene (TCE). In this study, our investigation on different culture conditions of this DPH-1 strain was extended
to find a more efficient and cost effective growth medium composition for this DPH-1 strain in bioremediation practices. Temperature
dependency of strain DPH-1 showed that the growth starting time and PCE degradation at 15°C was very slow compared to that
of 30°C, but complete PCE degradation occurred in both cases. For the proper utilization of strain DPH-1 in more cost effective
bioremediation practices, a simpler composition of an effective media was studied. One component of the culture medium, yeast
extract, had been substituted by molasses, which served as a good source of electron donor. The DPH-1 strain in the medium
containing molasses, in the presence of K2HPO4 and KH2PO4, showed identical bacterial multiplication (0.135 mg protein mL−1h−1) and PCE degradation rates (0.38 μM/h) to those of the yeast extract containing medium. |
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Keywords: | culture conditions PCE dechlorination Clostridium bifermentans DPH-1 molasses bioremediation |
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