Overexpression of a chloroplast-localized small heat shock protein OsHSP26 confers enhanced tolerance against oxidative and heat stresses in tall fescue |
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Authors: | Kyung-Hee Kim Iftekhar Alam Yong-Goo Kim Shamima Akhtar Sharmin Ki-Won Lee Sang-Hoon Lee Byung-Hyun Lee |
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Institution: | (1) Division of Applied Life Sciences (BK21), IALS, PMBBRC, Gyeongsang National University, Jinju, 660-701, Korea;(2) Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, 330-801, Korea |
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Abstract: | Small heat shock proteins are involved in stress tolerance. We previously isolated and characterized a rice cDNA clone, Oshsp26, encoding a chloroplast-localized small heat shock protein that is expressed following oxidative or heat stress. In this
study, we transferred this gene to tall fescue plants by an Agrobacterium-mediated transformation system. The integration and expression of the transgene was confirmed by PCR, Southern, northern,
and immunoblot analyzes. Compared to the control plants, the transgenic plants had significantly lower electrolyte leakage
and accumulation of thiobarbituric acid-reactive substances when exposed to heat or methyl viologen. The photochemical efficiency
of photosystem II (PSII) (Fv/Fm) in the transgenic tall fescue plants was higher than that in the control plants during heat
stress (42°C). These results suggest that the OsHSP26 protein plays an important role in the protection of PSII during heat
and oxidative stress in vivo. |
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