Chronological Changes in MicroRNA Expression in the Developing Human Brain |
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| Authors: | Michael P Moreau Shannon E Bruse Rebecka Jornsten Yushi Liu Linda M Brzustowicz |
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| Institution: | 1. Department of Genetics, Rutgers University, Piscataway, New Jersey, United States of America.; 2. Lovelace Respiratory Research Institute, Albuquerque, New Mexico, United States of America.; 3. Mathematical Sciences, University of Gothenburg and Chalmers University of Technology, Gothenburg, Sweden.; French National Center for Scientific Research – Institut de biologie moléculaire et cellulaire, France, |
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| Abstract: | ObjectiveMicroRNAs (miRNAs) are endogenously expressed noncoding RNA molecules that are believed to regulate multiple neurobiological processes. Expression studies have revealed distinct temporal expression patterns in the developing rodent and porcine brain, but comprehensive profiling in the developing human brain has not been previously reported.MethodsWe performed microarray and TaqMan-based expression analysis of all annotated mature miRNAs (miRBase 10.0) as well as 373 novel, predicted miRNAs. Expression levels were measured in 48 post-mortem brain tissue samples, representing gestational ages 14–24 weeks, as well as early postnatal and adult time points.ResultsExpression levels of 312 miRNAs changed significantly between at least two of the broad age categories, defined as fetal, young, and adult.ConclusionsWe have constructed a miRNA expression atlas of the developing human brain, and we propose a classification scheme to guide future studies of neurobiological function. |
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