Recombinant salmonella-based 4-1BBL vaccine enhances T cell immunity and inhibits the development of colorectal cancer in rats: in vivo effects of vaccine containing 4-1BBL |
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| Authors: | Jianxin Ye Ling Li Yuanting Zhang Xueguang Zhang Daming Ren Weichang Chen |
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| Affiliation: | 1.Department of Gastroenterology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China;2.Institute of Medical Biotechnology, Key Laboratory of Medicine and Clinical Immunology of Jiangsu Province, Soochow University, 215007, Suzhou, China;3.Institute of Genetics, State Key Laboratory of Genetic Engineering of Fudan University, 200433, Shanghai, China |
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| Abstract: | BackgroundImmunotherapy with vaccines is attractive for the treatment of cancer. This study is aimed at determining the effect of recombinant Salmonella (SL3261)-based 4-1BB ligand (4-1BBL) vaccine on the development of colorectal cancers and the potential immune mechanisms in rats.ResultsIn comparison with that in the PBS group, similar levels of 4-1BBL expression, the frequency of T cells, IFN-γ responses, and comparable numbers of tumors were detected in the SL3261 and SL3261C groups of rats. In contrast, significantly fewer numbers of tumors, increased levels of 4-1BBL expression in the spleens and colorectal tissues, higher frequency of peripheral blood and splenic CD3+CD25+ T cells, and stronger splenic T cell IFN-γ responses were detected in the SL3261R group of rats.ConclusionOur results indicated that vaccination with recombinant attenuated Salmonella harboring the 4-1BBL gene efficiently enhanced T cell immunity and inhibited the development of carcinogen-induced colorectal cancers in rats. |
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| Keywords: | Recombinant attenuated salmonella Colorectal cancer 4-1BBL T cell-based cellular immunity |
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