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Robust and Highly-Efficient Differentiation of Functional Monocytic Cells from Human Pluripotent Stem Cells under Serum- and Feeder Cell-Free Conditions
Authors:Masakatsu D Yanagimachi  Akira Niwa  Takayuki Tanaka  Fumiko Honda-Ozaki  Seiko Nishimoto  Yuuki Murata  Takahiro Yasumi  Jun Ito  Shota Tomida  Koichi Oshima  Isao Asaka  Hiroaki Goto  Toshio Heike  Tatsutoshi Nakahata  Megumu K Saito
Institution:1. Department of Clinilcal Application, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.; 2. Department of Fundamental Cell Technology, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.; 3. Department of Pediatrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.; 4. Department of Pediatrics, Yokohama City University Graduate School of Medicine, Yokohama, Japan.; University of Tampere, Finland,
Abstract:Monocytic lineage cells (monocytes, macrophages and dendritic cells) play important roles in immune responses and are involved in various pathological conditions. The development of monocytic cells from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) is of particular interest because it provides an unlimited cell source for clinical application and basic research on disease pathology. Although the methods for monocytic cell differentiation from ESCs/iPSCs using embryonic body or feeder co-culture systems have already been established, these methods depend on the use of xenogeneic materials and, therefore, have a relatively poor-reproducibility. Here, we established a robust and highly-efficient method to differentiate functional monocytic cells from ESCs/iPSCs under serum- and feeder cell-free conditions. This method produced 1.3×106±0.3×106 floating monocytes from approximately 30 clusters of ESCs/iPSCs 5–6 times per course of differentiation. Such monocytes could be differentiated into functional macrophages and dendritic cells. This method should be useful for regenerative medicine, disease-specific iPSC studies and drug discovery.
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