Cobalamin Analogues in Humans: A Study on Maternal and Cord Blood |
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| Authors: | Tore Forsingdal Hardlei Rima Obeid Wolfgang Herrmann Ebba Nexo |
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| Institution: | 1. Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark.; 2. Department of Clinical Chemistry, Faculty of Medicine, University Hospital of Saarland, Homburg/Saar, Germany.; Gentofte University Hospital, Denmark, |
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| Abstract: | BackgroundHaptocorrin (HC) carries cobalamin analogues (CorA), but whether CorA are produced in the body is unknown. All cobalamins (Cbl) to the foetus are delivered by the Cbl-specific protein transcobalamin (TC), and therefore analysis of cord serum for CorA may help to clarify the origin of CorA.MethodsHC-CorA were quantified in paired samples of cord serum from newborns and serum from mothers (n = 69).ResultsThe CorA-concentration was higher in cord serum (median = 380, range: 41–780 pmol/L) than in serum from the mothers (median = 160, range: 64–330 pmol/L), (p<0.0001). HPLC-analysis showed CorA-peaks with retention times of 13.5, 14,5 and 16.5 min in samples from both the mother and cord serum. The peak with retention time 16.5 min constituted 24% (mother) and 45% (cord serum) of the total amount CorA, and eluted as does dicyanocobinamide.ConclusionOur results support that CorA in the human body are derived from Cbl. |
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