Isolation and characterization of a stable l-arginine producer from continuous culture broth of Corynebacterium acetoacidophilum |
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| Affiliation: | 1. Key Laboratory of Marine Chemistry Theory and Technology of Ministry of Education, and College of Chemistry and Chemical Engineering, Ocean University of China, 238 Songling Road, Qingdao 266100, China;2. Laboratory for Biosensing, and Key Laboratory of Biofuels, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China;1. Microbial Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea;2. Department of Bioinformatics, University of Science and Technology, Daejeon, Republic of Korea;3. Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan;1. Department of Chemistry, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 11951, Saudi Arabia;2. Deanship of Scientific Research, Princess Nora Bint Abdul Rahman University, Saudi Arabia;3. Department of Chemistry, Faculty of Science, Jazan University, Jazan, Saudi Arabia;1. Institute for Biosensing, and College of Life Sciences, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China;2. Qingdao Institute of Bioenergy & Bioprocess Technology, and Key Laboratory of Bioenergy, Chinese Academy of Sciences, 189 Songling Road, Qingdao, 266101, China;3. College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, China;4. Department of Drug Metabolism and Analysis, School of Pharmacy, Medical College, Qingdao University, Qingdao, 266021, China;1. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;2. McKusick-Zhang Center for Genetic Medicine, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China;3. University of Chinese Academy of Sciences, Beijing 100049, China;4. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China;5. Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China;6. Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China |
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| Abstract: | In order to produce l-arginine efficiently, continuous culture was attempted using an l-arginine producing strain of Corynebacterium acetoacidophilum, MC-13. l-Arginine production by strain MC-13 decreased after shifting from fed-batch culture to continuous culture because various strains with reduced or no l-arginine productivity, including l-arginine auxotrophs, appeared in addition to the original l-arginine producer. Strain SC-190, isolated from the continuous culture broth of strain MC-13, produced l-arginine on cultivation in a stable fashion for more than 250 h. It is suggested that strain SC-190 acquired a lower susceptibility to the inhibition of growth and l-arginine production by l-arginine than the parental strain MC-13 due to this stabilization. |
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