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Influence of redox potential on methanation of methanol by Methanosarcina barkeri in Eh-stat batch cultures
Institution:1. IFTS, Rue Marcel Pagnol, 47510 Foulayronnes, France;2. I2M (CNRS UMR 5295), Univ. Bordeaux et Arts et Métiers ParisTech, 33400 Talence, France;1. Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan;2. Japan Oil, Gas and Metals National Corporation (JOGMEC), 2-10-1 Toranomon, Minato-ku, Tokyo 105-0001, Japan;3. Metals Technology Center, Japan Oil, Gas and Metals National Corporation (JOGMEC), 9-3 Furudate, Kosaka-kozan, Kosaka, Akita 017-0202, Japan
Abstract:The effect of Eh on the methanogenesis of methanol by Methanosarcina barkeri strain Fusaro was studied in pH-controlled anaerobic batch cultures at 37°C, in which the Eh of the culture medium was controlled by the addition of Ti(III)-citrate at values ranging from ?340 to ?520 mV. The changes in Eh revealed that the specific growth rate, μ, specific methane production rate, QCH4 and growth yield, YX/S were optimum under an Eh between ?430 and ?520 mV, while they decreased at the higher Eh of ?340 mV. The maximum values of QCH4 and μ under the optimum Eh condition were 210 ml CH4/g dry cell weight·h?1 and 0.11 h?1, respectively.
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