Isolation of l-methionine-enriched mutant of a methylotrophic yeast,Candida biodinii No. 2201 |
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| Institution: | 1. Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Kyoto 606, Japan;2. Department of Food Technology, Korea University, Seoul 132, Korea;1. Department of Pharmacy and Biotechnology, University of Bologna, Via Belmeloro 6, 40126, Bologna, Italy;2. Department of Chemistry “U. Schiff”, University of Florence, Via U. Schiff 6, 50019, Sesto Fiorentino, Florence, Italy;1. School of Chemical Engineering and Technology, State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin 300072, PR China;2. Collaborative Innovation Center of Chemistry Science and Engineering, Tianjin 300072, PR China;3. CNPC Greatwall Drilling Co. Ltd., Beijing 100101, PR China;4. Tianjin Hengbida Chemical Synthesis Co. Ltd., Tianjin 300072, PR China |
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| Abstract: | Six strains of methylotrophic yeast were examined for production of l-methionine-enriched cells. Candida biodinii (Kloeckera sp.) No. 2201, which accumulated 0.54 mg/g-dry cell weight (DCW) of free l-methionine (pool methionine), was selected as the parental strain for breeding l-methionine-rich mutants. Ethionine-resistant mutants were derived from the strain by UV irradation. A mutant strain, E500-78, which was resistant to 500 μg/ml of dl-ethionine, accumated 6.02 mg/g-DCW of pool methionine. The culture conditions for mutant strain E500-78 to increase pool methionine accumulation were o-ptimized. As a result, the mutant strain accumulated 8.80 mg/g-DCW of pool methionine and contained 16.02 mg/g-DCW total methionine. |
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