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Analysis of a promoter-like region in Flavobacterium which controls 6-aminohexanoic acid linear oligomer hydrolase gene expression in Escherichia coli |
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| Affiliation: | 1. Department of Chemistry and Chemical Biology, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4M1, Canada;2. Department of Biochemistry & Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4M1, Canada;1. Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany;2. Center for Biotechnology and Biomedicine, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany |
| Abstract: | The region located 6.0–6.2 kb upstream from a structural gene for 6-aminohexanoic acid linear oligomer hydrolase (EII) in Flavobacterium was found to be responsible for EII expression in Escherichia coli. In this region there existed a 50 bp long inverted repeat containing a sequence homologous to the E. coli promoter −35 and −10 regions. Introduction of deletions in this region resulted in decreases in EII activity to various levels. |
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