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Rapid DNA purification for restriction fragment length polymorphism analysis

Affiliation:	1. Iran University of Medical Sciences, Ali-Asghar Children’s Hospital, Tehran, Iran;2. Pediatric Infections Research Center, Mofid Children’s Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran;3. Department of Microbiology and Parasitology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran;4. Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran;5. Department of Pediatrics, University of Alberta, Edmonton, AB, Canada;1. Department of Neurosurgery, Oslo University Hospital–Rikshospitalet, Oslo, Norway;2. Faculty of Medicine, University of Oslo, Oslo, Norway

Abstract:	This paper describes a method for isolation of DNA from blood samples involving a rapid chemical disintegration of proteins with 8 M urea and with a minimum of exposure to phenol. The DNA is further desalted and purified on Sephadex G-25 prepacked disposable columns. DNA isolated in this way was pure enough to be immediately cleaved by restriction enzymes.

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