LC/MS profiling of flavonoid glycoconjugates isolated from hairy roots, suspension root cell cultures and seedling roots of Medicago truncatula |
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Authors: | Anna Staszk??w Barbara Swarcewicz Joanna Banasiak Dorota Muth Micha? Jasi??ski Maciej Stobiecki |
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Institution: | (1) Institute of Bioorganic Chemistry, PAS, Noskowskiego 12/14, 61-704 Poznań, Poland;(2) Faculty of Agronomy, University of Life Sciences, Wołyńska 35, 60-637 Poznań, Poland; |
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Abstract: | Hairy roots and suspension cell cultures are commonly used in deciphering different problems related to the biochemistry and
physiology of plant secondary metabolites. Here, we address about the issue of possible differences in the profiles of flavonoid
compounds and their glycoconjugates derived from various plant materials grown in a standard culture media. We compared profiles
of flavonoids isolated from seedling roots, hairy roots, and suspension root cell cultures of a model legume plant, Medicago truncatula. The analyses were conducted with plant isolates as well as the media. The LC/MS profiles of target natural products obtained
from M. truncatula seedling roots, hairy roots, and suspension root cell cultures differed substantially. The most abundant compounds in seedlings
roots were mono- and diglucuronides of isoflavones and/or flavones. This type of glycosylation was not observed in hairy roots
or suspension root cell cultures. The only recognized glycoconjugates in the latter samples were glucose derivatives of isoflavones.
Application of a high-resolution mass spectrometer helped evaluate the elemental composition of protonated molecules, such
as M + H]+. Comparison of collision-induced dissociation MS/MS spectra registered with a quadrupole time-of-flight analyzer for tissue
extracts and standards allowed us to estimate the aglycone structure on the basis of the pseudo-MS3 experiment. Structures of these natural products were described according to the registered mass spectra and literature data.
The analyses conducted represent an overview of flavonoids and their conjugates in different types of plant material representing
the model legume, M. truncatula. |
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