The contribution of protein kinase C and rho-kinase to the regulation of receptor-dependent contraction of arteries decreases with age independently of sympathetic innervation |
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| Authors: | S. V. Mochalov V. U. Kalenchuk D. K. Gainullina A. V. Vorotnikov O. S. Tarasova |
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| Affiliation: | (1) Biological Department, Moscow State University, Moscow, 119991, Russia;(2) Department of Fundamental Medicine, Moscow State University, Lomonosovskii pr. 31, korp. 5, Moscow, 119192, Russia;(3) Institute of Biomedical Problems, Russian Academy of Sciences, State Research Center of Russian Federation, Khoroshevskoe sh. 76A, Moscow, 123007, Russia |
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| Abstract: | The age-related dynamics of the activity of signaling pathways coupled with α1-adrenoreceptors and their dependence on sympathetic innervation of arterial smooth muscle has been studied. For this purpose, the effects of protein kinase C inhibitor (GF 109203X, 10?6 M and Rho-kinase inhibitor (Y27632, 10?5 M) on isometric contraction of the rat saphenous artery in response to the α1-adrenoreceptor agonist methoxamine were determined. The rats in the age of two weeks (with partially developed sympathetic innervation) had the lower vascular sensitivity to methoxamine than adult rats, but the effects of both inhibitors were more prominent. The denervation induced by excision of sympathetic ganglia in adult rats increased the arterial sensitivity to methoxamine but the sensitivity to inhibitors was unchanged. Thus, the postnatal development of the arterial smooth muscle is accompanied by diminution of the role of protein kinase C and Rho-kinase in the regulation of contraction, but these changes do not correlate with the changes in arterial sensitivity to α1-adrenergic stimulation and development of sympathetic innervation. |
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| Keywords: | smooth muscle methoxamine GF 109203X Y27632 postnatal development denervation |
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