Determination of glucose-fructose oxidoreductase activity in whole cells of Zymomonas mobilis |
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| Authors: | G S Erzinger M M Silveira M Vitolo R Jonas |
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| Institution: | (1) Centro de Desenvolvimento Biotecnológico, Caixa Postal 7151, 89239-970 Joinville, SC, Brazil;(2) Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Caixa Postal 66083, 05389-970 São Paulo, Brazil;(3) Gesellschaft für Biotechnologische Forschung mbh. Braunschweig, Germany |
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| Abstract: | A method for the determination of glucose-fructose oxidoreductase (GFOR) activity in whole cells of Zymomonas mobilis is described. The K
m and the theoretical V
max for GFOR were 192 g glucose.l-1 and 17 g gluconic acid.g-1 cell.h-1, respectively. The changes in enthalpy (31.1 kJ.mol-1), entropy (0.41 kJ.K-1), and Gibbs free energy (-97.5 kJ.mol-1) related to glucose to gluconic acid conversion were also determined. |
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| Keywords: | Gluconic acid glucose-fructose oxidoreductase sorbitol Zymomonas mobilis |
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