Subcellular distribution of hydrolase in germinating pea cotyledons |
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| Authors: | Nakano Masatoshi; Asahi Tadashi |
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| Institution: | Laboratory of Biochemistry, Faculty of Agriculture, Nagoya University Nagoya, Japan |
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| Abstract: | The subcellular distribution of hydrolases in germinating peacotyledons was investigated by differential and sucrose densitygradient centrifugations. Acid phosphatase was present in atleast two kinds of subcellular components. One was slightlyheavier than mitochondria, and the other was so light that itremained on the upper part of the gradient after sucrose densitygradient centrifugation. Protease was localized in the smooth-surfacedmicrosomal fraction which contained antimycin A-insensitiveNADH2-cytochrome c reductase and no RNA. Aryl esterase existedin a soluble form in the cytoplasm. (Received July 22, 1971; ) |
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