Diethylpyrocarbonate—An effective agent for the sterilization of different types of nutrient media |
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| Authors: | T Macek T Vaněk M Macková |
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| Institution: | (1) Department of Plant Tissue Cultures, Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo n.2, 166 10 Prague 6, Czech Republic;(2) Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, ICT Prague, Technická 3, 166 28 Prague 6, Czech Republic |
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| Abstract: | A simple and convenient method has been tested for the steriltzation of nutrient media for long-term cultivation of plant cells. Diethylpyrocarbonate is suitable for this task in concentrations about 1000 mg l-1 The cells cultivated for 15 subsequent passages on media treated by DPC had the same growth parameters, production pattern and ability to transform exogeneous organic compounds as did the controls. The method is suitable for the preparatian of both liquid and agar media, for stabilization of stock solutions and for sterilization of cultivation vessels and tubing.Abbreviations DPC
diethylpyrocarbonate
- medium MS
nutrient medium according to Murashige and Skoog
- NAA
naphtaleneacetic acid |
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| Keywords: | Diethylpyrocarbonate media sterilization plant cell cultures |
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