Genomic DNA amplification from a single bacterium |
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| Authors: | Raghunathan Arumugham Ferguson Harley R Bornarth Carole J Song Wanmin Driscoll Mark Lasken Roger S |
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| Affiliation: | Molecular Staging, Inc., New Haven, Connecticut, USA. |
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| Abstract: | Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying non-culturable microorganisms. |
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