Nerve growth factor in skeletal tissues of the embryonic chick |
| |
Authors: | Sally R. Frenkel Ladislao A. Guerra Ormond G. Mitchell Inder J. Singh |
| |
Affiliation: | (1) Department of Anatomy, New York University Dental Center, New York, USA;(2) Graduate School of Arts and Science, New York, USA;(3) Department of Anatomy, New York University Dental Center, 345 E. 24 St., 10010 New York, NY, USA |
| |
Abstract: | Summary This study demonstrates, via immunohistochemistry and bioassay, the presence of NGF in embryonic bone and cartilage of the chick. Embryos were killed on days 6–9 of incubation at 12 h intervals, and on days 10–18 at 24 h intervals. Paraffin-embedded sections of hind limbs or buds were immunostained with a polyclonal antibody against NGF and the biotin-avidin-horseradish peroxidase technique. Immunostaining was positive in both bone and cartilage, with cartilage staining more intensely. For bioassay, bones from the hind limbs of 9- and 12-day embryos were fast-frozen, lyophilized, and homogenized with Medium 199 (M199). Dorsal root ganglia from 8-day embryos were cultured for 24–36 h with rooster plasma, M199, and varying concentrations of bone homogenate. Significant neurite outgrowth was seen, with the greatest response elicited by 12-day bone homogenate. Addition of anti-NGF to the cultures abolished neurite outgrowth. The results indicate that NGF is present in cartilage and bone of the chick embryo; it may determine the density of sympathetic innervation to the developing skeletal tissues. |
| |
Keywords: | Nerve growth factor Osteogenesis Chick embryo |
本文献已被 SpringerLink 等数据库收录! |
|