| Bt水稻"克螟稻”花粉对家蚕生长发育的影响 |
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| 引用本文： | 王忠华,倪新强,徐孟奎,舒庆尧,夏英武.Bt水稻"克螟稻”花粉对家蚕生长发育的影响[J].遗传,2001,23(5):463-466. |
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| 作者姓名： | 王忠华 倪新强 徐孟奎 舒庆尧 夏英武 |
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| 作者单位： | 1。浙江大学原子核农业科学研究所，杭州 310029
2。浙江大学实验设备处，杭州 310031
3。浙江大学蚕学系，杭州 310029
1.Institute of Nuclear Agricultural Sciences,Zhejiang University,Hangzhou 310029,China;
2.Department of Experimental Equipment,Zhej iang University,Hangzhou 310031,China;
3.Department of sericulture,Zhejiang University,Hangzhou 310029,China |
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| 基金项目： | 受国家植物转基因专项(J2000–B–009),浙江省科委重大项目(991102004–002)和美国洛氏基金(2000FS020)共同资助 |
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| 摘 要： | 本试验以家蚕为供试对象研究Bt水稻"克螟稻”花粉对家蚕生长发育的影响.结果发现,与正常无花粉处理相比,无论是非Bt水稻花粉处理,还是Bt水稻花粉处理,对初孵家蚕幼虫的致死率无多大影响,而对家蚕的体重有较大影响,其中三龄期家蚕体重存在极显著差异.还发现Bt水稻花粉处理组家蚕在3龄时期大小很不一致,最轻体重为18.1mg,而最重体重为183.8mg.这是由于采用人工抖粉桑叶上花粉浓度不均匀造成的.鉴于实际生态条件下桑叶上的花粉浓度可能远远低于试验条件,因此,在实际稻桑共作环境下,Bt水稻"克螟稻”花粉对家蚕生长发育可能不会造成太大的影响。
Abstract:The effect on the development of silkworm larvae of Bt transgenic rice pollen containing cry1 Ab gene from Bacillus thuringiensis were investigated.Compared with normal treatment,mortality of newly hatched silkworm lar vae in either Bt rice pollen or susceptible rice pollen treatment were not significantly different,while the variances of silkworm larvae weight at third instar were significant at 0.01level.In addition,the weight of each silkworm larva at third instar in Bt rice pollen treatment showed a big difference,the biggest and smallest silkworm larvae were 183.8rug and 18.lmg respectively,which was probably caused by the difference of Bt rice pollen concentration on the mulberry leaves.As pollen concentration on the mulberry leaves in actual field was lower than in lab,the influence on the development of newly hatched silkworm larvae of Bt rice pollen is not likely significant in actual ecological cnvi ronment.
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| 关 键 词： | 生长发育 Key words Bt rice 家蚕 花粉 Bt转基因水稻 |
| 文章编号： | 0253-9772(2001)05-0463-04 |
| 修稿时间： | 2000年12月22 |
The Effect on the Development of Silkworm Larvae of Transgenic Rice Pollen with a Synthetic cry1Ab Gene from Bacillus thuringiensis |
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| WANG Zhong hua ,NI Xin qiang ,XU Meng kui ,SHU Qing yao ,XIA Ying wu.The Effect on the Development of Silkworm Larvae of Transgenic Rice Pollen with a Synthetic cry1Ab Gene from Bacillus thuringiensis[J].Hereditas,2001,23(5):463-466. |
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| Authors: | WANG Zhong hua NI Xin qiang XU Meng kui SHU Qing yao XIA Ying wu |
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| Institution: | WANG Zhong hua 1,NI Xin qiang 2,XU Meng kui 3,SHU Qing yao 1,XIA Ying wu 1 |
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| Abstract: | The effect on the development of silkworm larvae of Bt transgenic rice pollen containing cry1 Ab gene from Bacillus thuringiensis were investigated.Compared with normal treatment,mortality of newly hatched silkworm larvae in either Bt rice pollen or susceptible rice pollen treatment were not significantly different,while the variances of silkworm larvae weight at third instar were significant at 0.01level.In addition,the weight of each silkworm larva at third instar in Bt rice pollen treatment showed a big difference,the biggest and smallest silkworm larvae were 183.8mg and 18.1mg respectively,which was probably caused by the difference of Bt rice pollen concentration on the mulberry leaves.As pollen concentration on the mulberry leaves in actual field was lower than in lab,the influence on the development of newly hatched silkworm larvae of Bt rice pollen is not likely significant in actual ecological environment. |
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| Keywords: | Bt rice pollen Bombyx mori L development |
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