Identification of three chitin synthase genes in the dimorphic fungal pathogenSporothrix schenckii |
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| Authors: | Chua Steven S. Momany Michelle Mendoza Leonel Szaniszlo Paul J. |
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| Affiliation: | (1) Department of Microbiology, University of Texas at Austin, 78712-1095 Austin, TX, USA;(2) Division of Biological Sciences, University of Texas at Austin, Austin, TX, USA;(3) Present address: Department of Cell Biology, Baylor College of Medicine, One Baylor Plaza, 77030 Houston, TX;(4) Present address: Department of Biological Sciences, Purdue University, 47907 West Lafayette, IN |
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| Abstract: | Degenerate PCR primers were used to amplify a 600-bp conserved gene region for chitin synthases from genomic DNA ofSporothrix schenckii, a dimorphic fungal pathogen of humans and animals. Three chitin synthase gene homologs were amplified as shown by DNA sequence analysis and by Southern blotting experiments. Based on differences among the predicted amino acid sequences of these homologs, each was placed within one of three different chitin synthase classes. Phylogenies constructed with the sequences and the PAUP 3.1.1. program showed thatS. schenckii consistently clustered most closely withNeurospora crassa in each of the three chitin synthase classes. These findings are significant because the phylogenies support by a new method the grouping of the imperfect fungusS. schenckii with the Pyrenomycetes of the Ascomycota. |
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