Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems |
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| Authors: | Chien Wei Ooi Beng Ti Tey Siew Ling Hii Siti Mazlina Mustapa Kamal John Chi Wei Lan Arbakariya Ariff Tau Chuan Ling |
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| Affiliation: | 1. Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;2. Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;3. Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, 53300 Setapak, Kuala Lumpur, Malaysia;4. Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;5. Department of Chemical Engineering and Materials Science, College of Engineering, Yuan Ze University, Taiwan |
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| Abstract: | Alcohol/salt-based aqueous two-phase systems (ATPSs) were used to recover lipase derived from Burkholderia pseudomallei (B. pseudomallei). Nine biphasic systems, comprised of an alcohol-based top phase (ethanol, 2-propanol and 1-propanol) and a salt-based bottom phase (ammonium sulfate, potassium phosphate and sodium citrate), were evaluated for their effectiveness in lipase recovery. The stability of lipase in each of the solutions was tested, and phase diagrams were constructed for each system. The optimum partition efficiency for the purification of lipase was obtained in an ATPS of 16% (w/w) 2-propanol and 16% (w/w) phosphate in the presence of 4.5% (w/v) NaCl. The purified lipase had a purification factor of 13.5 and a yield of 99%. |
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| Keywords: | Lipase Aqueous two-phase system Alcohol/salt system Purification Burkholderia pseudomallei |
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