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Quinol oxidation by c-type cytochromes: structural characterization of the menaquinol binding site of NrfHA
Authors:Rodrigues Maria Luisa  Scott Kathryn A  Sansom Mark S P  Pereira Inês A C  Archer Margarida
Institution:1 Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, ITQB-UNL, Av. República, 2780-157 Oeiras, Portugal
2 Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Abstract:Membrane-bound cytochrome c quinol dehydrogenases play a crucial role in bacterial respiration by oxidizing menaquinol and transferring electrons to various periplasmic oxidoreductases. In this work, the menaquinol oxidation site of NrfH was characterized by the determination of the X-ray structure of Desulfovibrio vulgaris NrfHA nitrite reductase complex bound to 2-heptyl-4-hydroxyquinoline-N-oxide, which is shown to act as a competitive inhibitor of NrfH quinol oxidation activity. The structure, at 2.8-Å resolution, reveals that the inhibitor binds close to NrfH heme 1, where it establishes polar contacts with two essential residues: Asp89, the residue occupying the heme distal ligand position, and Lys82, a strictly conserved residue. The menaquinol binding cavity is largely polar and has a wide opening to the protein surface. Coarse-grained molecular dynamics simulations suggest that the quinol binding site of NrfH and several other respiratory enzymes lie in the head group region of the membrane, which probably facilitates proton transfer to the periplasm. Although NrfH is not a multi-span membrane protein, its quinol binding site has several characteristics similar to those of quinone binding sites previously described. The data presented here provide the first characterization of the quinol binding site of the cytochrome c quinol dehydrogenase family.
Keywords:HQNO  2-heptyl-4-hydroxyquinoline-N-oxide  CG  coarse-grained  MD  molecular dynamics  MQ7  menaquinone-7  PCP  pentachlorophenol  PEG  polyethylene glycol  DMN  2  3-dimethyl-1  4-naphthoquinone
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