Molecular cloning of pea mRNAs encoding a shoot-specific polypeptide and light-induced polypeptides |
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Authors: | Sacco C. De Vries Marco C. Harmsen Martin T. R. Kuiper Hans J. M. Dons Joseph G. H. Wessels |
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Affiliation: | (1) Department of Developmental Plant Biology, Biological Centre, State University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands |
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Abstract: | Summary The molecular cloning of cDNA corresponds to pea seedling mRNA sequences encoding a shoot-specific polypeptide, the small subunit of the ribulose 1,5 biphosphate carboxylase and a component of the light-harvesting chlorophyll a/b complex is described. cDNA prepared from polysomal poly(A)RNA of light-grown shoots was enriched for shoot-specific and light-induced sequences by heterologous liquid hybridization with mercurated polysomal poly(A)RNA of dark-grown roots, followed by sulfhydryl chromatography. Cloned shoot-specific sequences were identified by 2D electrophoretic analysis of hybrid release translation products. The cloned shoot-specific sequence corresponded to a mRNA of 850 nt present both in light-and dark-grown shoots, and produced anin vitro translation product of Mr27 500 and isoelectric point of 4.7. |
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Keywords: | cDNA cloning light-harvesting chlorophyll a/b protein Pisum shoot-specific polypeptide small subunit ribulose 1,5 biphosphate carboxylase |
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