Sphingobacterium pakistanensis sp. nov., a novel plant growth promoting rhizobacteria isolated from rhizosphere of Vigna mungo |
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Authors: | Iftikhar Ahmed Muhammad Ehsan Yeseul Sin Jayoung Paek Nauman Khalid Rifat Hayat Young H Chang |
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Institution: | 1. National Culture Collection of Pakistan (NCCP), National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Center (NARC), Park Road, Islamabad, 45500, Pakistan 2. Korean Collection for Type Cultures, Biological Resources Centre, KRIBB, 52 Eoeun-dong, Taejon, 305-806, Republic of Korea 4. Department of Global Agricultural Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-Ku, Tokyo, 113-8657, Japan 3. Department of Soil Science & SWC, PMAS Arid Agriculture University, Rawalpindi, Pakistan
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Abstract: | The taxonomic status of a bacterium, strain NCCP-246T, isolated from rhizosphere of Vigna mungo, was determined using a polyphasic taxonomic approach. The strain NCCP-246T can grow at 16–37 °C (optimum 32 °C), at pH ranges of 6–8 (optimum growth occurs at pH 7) and in 0–4 % (w/v) NaCl. Phylogenetic analysis based upon on 16S rRNA gene sequence comparison revealed that strain NCCP-246T belonged to genus Sphingobacterium. Strain NCCP-246T showed highest similarity to the type strain of Sphingobacterium canadense CR11T (97.67 %) and less than 97 % with other species of the genus. The DNA–DNA relatedness value of strain NCCP-246T with S. canadense CR11T and Sphingobacterium thalpophilum JCM 21153T was 55 and 44.4 %, respectively. The chemotaxonomic data revealed the major menaquinone as MK-7 and dominant cellular fatty acids were summed feature 3 C16:1 ω7c/C16:1 ω6c] (37.07 %), iso-C15:0 (28.03 %), C16:0 (11.85 %), C17:0 cyclo (8.84 %) and C14:0 (2.42 %). The G+C content of the strain was 39.2 mol%. On the basis of DNA–DNA hybridization, phylogenetic analyses, physiological and, biochemical data, strain NCCP-246T can be differentiated from the validly named members of genus Sphingobacterium and thus represents as a new species, for which the name, Sphingobacterium pakistanensis sp. nov. is proposed with the type strain NCCP-246T (= JCM18974 T = KCTC 23914T). |
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