Lectin like properties and differential sugar binding characteristics of C-reactive proteins purified from sera of normal and pollutant induced Labeo rohita |
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Authors: | Chitra Mandal Sukanya Sinha Chhabinath Mandal |
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Institution: | (1) Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Calcutta -, 700 032, India |
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Abstract: | Different forms of C-reactive proteins (CRPs) have been purified to electrophoretic homogeneity from the sera of Labeo rohita confined in freshwater (CRPN) and water polluted with nonlethal doses of cadmium (CRPCd) or mercury (CRPHg). CRPNemsp4 ], CRPCdemsp4 ], and CRPHg show remarkable differences in their electrophoretic mobility but exhibit strong immunological cross reactivity. All these CRPs exhibit variable agglutination properties with erythrocytes from diverse sources in presence of Ca+2, which could be inhibited by a variety of sugars showing specificity for galactose. Inhibition results show that the potency of galactose as an inhibitor increases about 4 fold in the process of transformation of CRPN to CRPCd and CRPHgemsp4 ]. In case of CRPNemsp4 ], Gal (1 1) Gal and oNO2 phenyl -Gal show highest inhibitory potency while oNO2-phenyl -Gal is the most potent inhibitor for CRPCd and CRPHg but the potency of Gal (1 1) Gal reduced drastically. 6-phosphate D-Gal and stachyose are 20 times weaker inhibitors than D-Gal for induced CRP mediated agglutination, in contrast, these sugars are only 6 times weaker for CRPNemsp4 ]. Dissociation constants of the binding of CRPN with phosphoryl choline (PC) and galactose are about 9emsp4 ]mM and PC binding causes a change in the and conformations of these CRPs. |
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Keywords: | C-reactive protein pollutant agglutinin galactose binding lectin fish Labeo rohita |
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