Intracellular localization of prenyltransferases of isoflavonoid phytoalexin biosynthesis in bean and soybean |
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Authors: | David R Biggs Roland Welle Hans Grisebach |
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Institution: | 1. Lehrstuhl für Biochemie der Pflanzen, Institut für Biologie II der Albert Ludwigs-Universit?t, Sch?nzlestrasse 1, D-7800, Freiburg, Germany
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Abstract: | The intracellular localization of prenyltransferases involved in the biosynthesis of the phytoalexins glyceollin in soybean
(Glycine max L.) and phaseollin in French bean (Phaseolus vulgaris L.) has been investigated. By sucrose- and Percoll-gradient centrifugation of microsomes of an elicitor-challenged soybean
cell culture, the membranes containing prenyltransferase were separated from the endoplasmic reticulum and shown to be lighter
in density. In a continuous Percoll gradient the peak of prenyltransferase activity coincided with the peak of galactolipid
synthesis, as determined by incorporation of uridine 5′-diphospho-14C]galactose (UDP-14C]galactose). Intact chloroplasts isolated from cupricchloride-treated bean leaves contained both prenyltransferase and UDP-galactose
transferase activity. Both activities increased during chloroplast isolation. Fractionation of swollen chloroplasts on a discontinuous
sucrose gradient showed prenyltransferase and UDP-galactose transferase activity in the envelope membrane subfraction. It
is concluded that in both plants prenyltransferase is located in the envelope membrane of plastids.
Dedicated to Professor Hans Mohr on the occasion of his 60th birthday |
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Keywords: | Glycine (phytoalexin synthesis) Isoflavonoid phytoalexins Phaseolus (phytoalexin synthesis) Phytoalexin synthesis Plastid envelope Prenyltransferase (localization) |
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