Variation with age in the numbers of ovulated ova and follicles of Wistar-Imamichi adult rats superovulated with eCG-hCG. |
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Authors: | Satosi Kagabu Motoaki Umezu |
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Institution: | Faculty of Agriculture, Yamaguchi University, Yoshida, Yamaguchi, Japan. |
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Abstract: | There are large variations with age in the number of ovulated ova found in superovulated female Wistar-Imamichi rats. In this study we investigated the numbers of ovulated ova and follicles with the aim of developing a superovulation technique that minimises variations. We also examined the number of non-atretic follicles in untreated rats aged 7-14 weeks, for each week of age. The numbers of 250-549 microm non-atretic follicles in untreated rats and the numbers of ovulated ova in superovulated rats both reached a peak at 12 weeks of age. The coefficients of variation for both follicle numbers and ova numbers changed with each week of age, reaching a maximum at 9 weeks of age and a minimum at 12 weeks of age. In order to achieve stable numbers of ova from superovulated rats, satisfactory results will be achieved using 12-week-old rats, minimising individual variations, with high numbers of ova. |
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