涎腺腺样囊性癌细胞蛋白激酶C的活性阻抑对其侵袭能力的影响

用蛋白激酶Ｃ（ＰＫＣ） 抑制剂Ｈ７ 作用于人涎腺腺样囊性癌ＳＡＣＣ８３ 系， 利用扫描电镜观察对人羊膜基底膜侵袭能力的影响。癌细胞接种于羊膜基底膜表面４８－ ７２ 小时后， 在扫描电镜下可以观察到对照组癌细胞侵袭现象， 侵袭的细胞伸出丝状伪足且侵入基底膜内， 伪足周围的基底膜断裂和被溶解， 而实验组癌细胞侵袭现象很难发现。细胞附于基底膜表面， 细胞表面较光滑且无明显伪足伸出， 基底膜无明显受损改变。实验结果表明， 降低涎腺腺样囊性癌细胞的ＰＫＣ活性可明显降低其侵袭能力， ＰＫＣ与涎腺腺样囊性癌的侵袭能力有密切的关系

THE SUPRESSION EFFECT OF PROTEIN KINASE C ACTIVITY ON INVASIVE CAPACITY OF SALIVARY ADENOID CYSTIC CARCINOMA CELLS

Human salivary adenoid carcinoma cell line SACC 83 was treated with protein kinase C inhibitor H7, and its invasive capacity to amniotic basement membrane was observed with SEM, 48-72 hrs after seeding, the invasive phenomena of SACC 83 cells could be observed in the control group. Invasive carcinoma cells extended silk like pseudopodia and invaded the inner part of basement membrane. Basement membrane surrounding pseudopodia was dissolved and distroyed. The invasive phenomena of the carcinoma cells were seldom found in experimental group. The surface of the cells was smooth and without pseudopodia, the basement membrane was not distinctly dissolved. The results showed that the inhibition of protein kinase C activity of salivary adenoid cystic carcinoma cells could reduce invasive capacity of the cells. There was close relationship between protein kinase C and the invasive capacity of salivary adenoid cystic carcinoma.