A novel,thermostable manganese-containing superoxide dismutase from <Emphasis Type="Italic">Bacillus licheniformis</Emphasis> |
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| Authors: | Ivanka Petrova Boyadzhieva Miroslava Atanasova Elka Emanuilova |
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| Institution: | (1) Department of Extremophilic Bacteria, Institute of Microbiology, Bulgarian Academy of Sciences, 26, Acad. G. Bonchev Str., 1113 Sofia, Bulgaria;(2) Present address: Department of Molecular Biology and Biotechnology, ANFACO-CECOPESCA, Crta. Colegio Universitario 16, 36310 Vigo, Pontevedra, Spain |
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| Abstract: | A new, thermostable superoxide dismutase (SOD) from Bacillus licheniformis M20, isolated from Bulgarian mineral springs, was purified 11-fold with 11% recovery of activity. From native PAGE and SDS-PAGE,
the enzyme was composed of two subunits of 21.5 kDa each. The SOD was inhibited only by NaN3, which suggested that this SOD is of the manganese superoxide dismutase type. The purified enzyme had maximum activity at
pH 8 and 55°C. The half-life of the SOD was 10 min at 95°C. |
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