Two glucuronoyl esterases of <Emphasis Type="Italic">Phanerochaete chrysosporium</Emphasis> |
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Authors: | Miroslava ?uranová Silvia ?pániková Han A B Wösten Peter Biely Ronald P de Vries |
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Institution: | (1) Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia;(2) Microbiology, Department of Biology, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands |
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Abstract: | The white-rot fungus Phanerochaete chrysosporium produces glucuronoyl esterase, a recently discovered carbohydrate esterase, during growth on sugar beet pulp. Two putative
genes encoding this enzyme, ge1 and ge2, were isolated and cloned. Heterologous expression in Aspergillus vadensis, Pycnoporus cinnabarinus and Schizophyllum commune resulted in extracellular glucuronoyl esterase activity, demonstrating that these genes encode this enzymatic function. The
amino acid sequence of GE1 was used to identify homologous genes in the genomes of twenty-four fungi. Approximately half of
the genomes, both from ascomycetes and basidiomycetes, contained putative orthologues, but their presence could not be assigned
to any of fungal class or subclass. Comparison of the amino acid sequences of identified and putative glucuronoyl esterases
to other types of carbohydrate esterases (CE) confirmed that they form a separate family of CEs. These enzymes are interesting
candidates for biotechnological applications such as the separation of lignin and hemicellulose. |
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Keywords: | Glucuronoyl esterase Phanerochaete chrysosporium Lignin-carbohydrate complex |
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