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A sensitive measurement of oxygen uptake rate using a optochemical sensor |
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| Authors: | Chung-Ming Chi Leah N. Frautschy Frank B. Cerra Wei-Shou Hu |
| Affiliation: | (1) Department of Chemical Engineering and Materials Science, University of Minnesota, 55455 Minneapolis, MN, USA;(2) Department of Surgery, University of Minnesota, 55455 Minneapolis, MN, USA |
| Abstract: | Summary The measurement of the specific oxygen uptake rate (OUR) of slow growing organisms using a small sample size is often hampered by the consumption of oxygen by the electrode used. Using a optochemical sensor we measured the OUR of carrot embryos with approximately 1000 cell clusters and of hybridoma with approximately 106 cells. An OUR as low as 0.02  mol/h can be accurately measured. |
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