Regioselective carboxylation of catechol by 3,4-dihydroxybenzoate decarboxylase of <Emphasis Type="Italic">Enterobacter cloacae</Emphasis> P |
| |
Authors: | Toyokazu Yoshida Yuki Inami Tsuyoshi Matsui Toru Nagasawa |
| |
Institution: | (1) Department of Biomolecular Science, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan |
| |
Abstract: | 3,4-Dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 was induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic
acid to the culture medium. After stabilizing the enzyme activity by adding 5 mM dithiothreitol and 20 mM Na2S2O3 to a cell-free extract, catechol at 50 mM was carboxylated in the presence of 3 M KHCO3 to 3,4-dihydroxybenzoic acid with a molar conversion ratio of 28% after 14 h at 30°C. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|