Evaluation of functional gene enrichment in a soil metagenomic clone library |
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| Authors: | Demanèche Sandrine David Maude M Navarro Elisabeth Simonet Pascal Vogel Timothy M |
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| Affiliation: | Environmental Microbial Genomics Group, Laboratoire AMPERE, UMR CNRS 5005, Ecole Centrale de Lyon, Université de Lyon, 36 avenue Guy de Collongue 69134 Ecully France. |
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| Abstract: | We evaluated the use of mixed oligonucleotide probes hybridized to metagenomic clones spotted on high density membranes. The pooled probes included oligonucleotides designed for genes associated with denitrification, antibiotic resistance, and dehalogenation among others. Pyrosequence comparison between the clones and the original DNA demonstrated the utility of clone screening with pooled probes. |
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