Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity |
| |
Authors: | Xuejiao Zhang Cheng Li Huiling Gao Hiroaki Nabeka Tetsuya Shimokawa Hiroyuki Wakisaka Seiji Matsuda Naoto Kobayashi |
| |
Institution: | (1) Molecular and Cellular Biology, The Beatson Institute for Cancer Research, Glasgow, United Kingdom, G61 1BD; |
| |
Abstract: | We investigated the effects of Rho-associated kinase (ROCK) on migration and cytoskeletal organization in primary human osteoblasts
and Saos-2 human osteosarcoma cells. Both cell types were exposed to two different ROCK inhibitors, Y-27632 and HA-1077. In
the improved motility assay used in the present study, Y-27632 and HA-1077 significantly increased the migration of both osteoblasts
and osteosarcoma cells on plastic in a dose-dependent and reversible manner. Fluorescent images showed that cells of both
types cultured with Y-27632 or HA-1077 exhibited a stellate appearance, with poor assembly of stress fibers and focal contacts.
Western blotting showed that ROCK inhibitors reduced myosin light chain (MLC) phosphorylation within 5 min without affecting
overall myosin light-chain protein levels. Inhibition of ROCK activity is thought to enhance the migration of human osteoblasts
through reorganization of the actin cytoskeleton and regulation of myosin activity. ROCK inhibitors may be potentially useful
as anabolic agents to enhance the biocompatibility of bone and joint prostheses. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|